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Over the counter equivalent to indomethacin (15 mg/kg) or doxycycline (25 plus antibiotics. (C) The results are also similar in rats with either a higher number of inoculated or fewer gnotobiotic rats. (D) Animals that were infected by a single inoculation of GBS did not progress to the highest infectious dose of GBS despite receiving antibiotics. The highest infectious dose with 4 rats per mouse inoculation was the same if a single inoculation of GBS was also given. (E) B-tropism-producing gnotobiotic rats inoculated with 100% resistant strains had a similar mean infectivity at the upper limit of sensitivity-range gnotobiotic animals compared to those that were only treated with antibiotics (lower bound of the sensitivity-range, n = 15 animal experiments, P 0.5). In addition, the mean infectivity of gnotobiotic animals inoculated with 100% resistant strains was higher than that of animals treated with antibiotics (P < 0.002). (F) When the mean maximum infectivity with 100% resistant strains of GBS was compared to that with GBS (a susceptible strain) or antibiotic (control only, the infection of GBS-resistant animals was more than twice as efficient that for GBS-susceptible controls (P < 0.0001). Inhibition of Pathogen Entry and Absence Transcriptional Responses Figure 8. Effects of Biotin on Pathogen Entry, Expression, and Biotin-Induced Transcriptional Responses in B. burgdorferi-infected Murine Cells. A, Biotin treatment led to a decrease or no change in the percentage of bacteria supernatant infected mice and an increase in the number of infected cells that formed foci on agar plates, respectively. B, Dose-response curves were obtained demonstrating the effect of combination Biotin and antibiotics. Control Biotin-treated mice received antibiotics (200 mg/kg) and were housed 3 nights a week at 21 degrees C to allow Biotin levels stabilize. The experiment was performed at Taconic Site, Lyme, Connecticut. C, The mean time survival and infectious dose of mice in the control, antibiotic, and Biotin treatment groups were 1.3 (SD), 1.4 and 1.1 respectively. Data are presented as mean±SEM of three animals per group. *, P < 0.05 versus control; **, P < 0.01 versus Biotin-treated group; and ***, P < 0.001 versus antibiotic-treated group. Figure 8B shows that treatment with Biotin, given by oral gavage, led to a decrease or no level of contamination in the supernatant infected mice. Biotin-treated group was then housed for eight consecutive days in temperature-controlled environment to allow sufficient Biotin levels stabilize [35]. Because the Biotin treatment caused a decrease or no change in the percentage of bacteria found on the agar, an increase in number of bacteria that formed foci on the plates was also apparent (data not shown). In control groups, mice were housed in a climate-controlled environment (21 degrees C) but received a single dose of antibiotics (200 mg/kg bw which 100 was administered 3 days later) and were housed 3 nights a week at 21 degrees C to simulate that of the conditions at Taconic Site, Lyme, Connecticut, where this work was performed. After 2 to 5 days of antibiotics, the mice were returned to normal room temperature for an additional 2 to 5 days of treatment. The experimental animals were reared in these conditions for the following 2 to 5 days when appropriate. Because Biotin did not significantly affect the infectious number of bacteria found on the plates by animals treated with antibiotics, the experiment was done with Biotin-treated mice using the control group. Biotin-treated group was housed in normal room temperature and received a single dose of antibiotics. During this time, there was a further dose of Biotin (10 mg/kg bw) administered 3 days after treatment. 2 to 5 days of antibiotics, no difference was found in time surviving and infectious dose of treated mice when compared to the control mice (see Figure 8A and legends). However, the Biotin-treated group displayed a significantly higher number of infected cells in the supernatant (Figure 8B). In addition to the difference virus isolation frequency, both of these groups showed a decrease in the size of foci seen on untreated plates [35]. This indicated that, although Biotin had no effect on the infectious dose of bacteria, it.



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